Biotransformaciones por hongos endófitos aislados de plantas medicinales tradicionales ecuatorianas: conectando la etnomedicina con la biotecnología

Laura  Scalvenzi Foglia

doi:10.59410/RACYT-v01n03ep06-0020

Authors

Laura Scalvenzi Universidad Estatal Amazónica https://orcid.org/0000-0001-6015-9871

DOI:

https://doi.org/10.59410/RACYT-v01n03ep06-0020

Keywords:

ethnomedicine, Amazonian plant, Andean plants, endophyte, endophytic fungi, biotechnology, biotransformation

Abstract

Ecuador, a small country with diverse ecosystems in the Amazon, Andes and Pacific coastal regions is considered one of the 17 "megadiverse” countries, and the native ethnic groups and rural communities have a strong ethnomedical tradition in the use of native plants in healing. Traditional ethnobotanical knowledge can be used to guide biotechnological research on medicinal plants, even when the new application is an innovation only distantly related to the traditional use. Based on ethnomedicalknowledge of indigenous communities, the following plants from the Amazon and Andes regions were chosen for investigation: Piper aduncum (Piperaceae), Maytenus macrocarpa (Celastraceae), Schinus molle (Anacardiaceae), Tecoma stans (Bignoniaceae) and Myrcianthes hallii (Myrtaceae). The research was focused on (i) assesing the presence of endophytic fungi in the selected plants, (ii) isolating and subculturing in vitro pure endophytic strains, (iii) assessing the biotransformation capacity of the isolated endophytes on pure compounds (intermediates of pharmaceutical synthesis). The following compounds were chosen as substrate models for biotransformations: (+/-)-cis-bicyclo[3.2.0]hept-2-en-6-one, acetophenone, 1-indanone, 2-furyl methyl ketone, 2-methylcyclopentanone, 2- methylcyclohexanone, 2-methoxycyclohexanone. A total of 364 fungal strains were isolated in vitro; among these, five strains performed biotransformations on acetophenone to (S)-1-phenylethanol, with important yields (78-97%) and enantiomeric excess (78-100%). Three strains also yielded phenols, probably by enzymatic reactions (Baeyer-Villiger oxidations). Fifteen fungal strains yielded the lactones (-)-(1S,5R)-2-oxabicyclo[3.3.0]oct-6-en-3-one and (-)-(1R,5S)-3- oxabicyclo[3.3.0]oct-6-en-2-one from (+/-)-cis-bicyclo[3.2.0]hept-2-en-6-one, probably as result of monooxygenase activation.

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